FREE delivery to all EXCLUSIVE BOOKS stores nationwide. FREE delivery to your door on all orders over R450. Excludes all international deliveries.

Recombinant Protein Production in Yeast

Roslyn M. Bill
    Product form
      FORMAT: Hardback

      R 5,448.94 Price and availability exclusive to website

      YOU COULD EARN 5,448.94 FUTURE RETAIL DISCOUNTS.
      ESTIMATED DELIVERY: Approx. 10 - 15 Business Days
      BUY NOW PAY LATER
      From R 908.15 per month!
      3x monthly payments of R 1,816.31 with
      4x fortnightly payments of R 1,362.23 with
      In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
      Format: Hardback CONTRIBUTORS: Roslyn M. Bill EAN: 9781617797699 COUNTRY: United States PAGES: WEIGHT: 693 g HEIGHT: 254 cm
      PUBLISHED BY: Humana Press Inc. DATE PUBLISHED: 2012-03-28 CITY: GENRE: MEDICAL / Genetics WIDTH: 178 cm SPINE:

      Book Themes:

      Medical genetics

      Customer Reviews

      Be the first to write a review
      0%
      (0)
      0%
      (0)
      0%
      (0)
      0%
      (0)
      0%
      (0)
      In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
      Format: Hardback CONTRIBUTORS: Roslyn M. Bill EAN: 9781617797699 COUNTRY: United States PAGES: WEIGHT: 693 g HEIGHT: 254 cm
      PUBLISHED BY: Humana Press Inc. DATE PUBLISHED: 2012-03-28 CITY: GENRE: MEDICAL / Genetics WIDTH: 178 cm SPINE:

      Book Themes:

      Medical genetics

      Customer Reviews

      Be the first to write a review
      0%
      (0)
      0%
      (0)
      0%
      (0)
      0%
      (0)
      0%
      (0)

      Recently viewed products

      Login

      Forgot your password?

      Don't have an account yet?
      Create account