Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. 

Fatma SarsuPlant Breeding and Genetics SectionJoint FAO/IAEA Division Vienna, Austria Abdelbagi M.A. GhanimPlant Breeding and Genetics Laboratory Joint FAO/IAEA Division Vienna, Austria Priyanka DasSchool of Life SciencesJawaharlal Nehru UniversityNew Delhi, India Rajeev N BahugunaSchool of Life SciencesJawaharlal Nehru UniversityNew Delhi, India Paul Mbogo Kusolwa Sokoine University of AgricultureMorogoro, United Republic of Tanzania Muhammed Ashraf Nuclear Institute for Agriculture and Biology (NIAB)Faisalabad, Pakistan  Sneh Lata Singla-Pareek Plant Molecular Biology GroupInternational Centre for Genetic Engineering and BiotechnologyNew Delhi, India Ashwani PareekSchool of Life SciencesJawaharlal Nehru UniversityNew Delhi, India Brian P. ForsterPlant Breeding and Genetics Laboratory Joint FAO/IAEA DivisionInternational Atomic Energy Agency Vienna, Austria Ivan Ingelbrecht Plant Breeding and Genetics Laboratory Joint FAO/IAEA Division International Atomic Energy Agency Vienna, Austria