Gene Expression Profiling

Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.  In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.

From the reviews of the second edition:“This beautifully illustrated book on gene expression profiling provides detailed protocols for a broad range of techniques … for the analysis of gene expression at the DNA, RNA, and protein levels. … Although written for researchers, the book will be helpful for graduate students and others interested in learning about gene expression. … This is a valuable resource on methods and protocols necessary to understand the changes in gene expression in normal and pathological states in pursuit of better treatments.” (Omer Iqbal, Doody’s Book Reviews, April, 2012)