Transforming Plant Genomes through CRISPR/Cas with Precision

The advent of CRISPR/Cas technology has ushered plant science into an era defined by precision, efficiency, and unprecedented possibility. Once constrained by lengthy breeding cycles and limited genetic resolution, plant genome engineering today stands transformed by a tool that allows targeted, predictable, and heritable modifications. This book volume has been conceived to capture both the scientific depth and the practical relevance of this revolutionary technology.The book brings together contributions from leading researchers who have shaped, and continue to shape, the CRISPR landscape in plant systems. Each chapter has been carefully curated to provide a balanced synthesis of foundational principles, methodological advances, and real-world applications. Readers will find comprehensive discussions on CRISPR/Cas variants, guide RNA design, delivery strategies, and editing outcomes, alongside emerging approaches such as base editing, prime editing, and multiplex genome engineering.This book is intended for researchers, educators, and advanced students seeking both conceptual clarity and practical insight. It is our hope that this volume will serve as a reliable reference, stimulate interdisciplinary dialogue, and inspire the next generation of plant biotechnologists to harness CRISPR/Cas for the benefit of global food security and environmental sustainability.

Ankur Bhardwaj works as Assistant Professor in the Department of Biotechnology, Shri Vaishnav Institute of Sciences, Shri Vaishnav Vidyapeeth Vishwavidyalaya, Indore, India. He holds a PhD (2021) in Molecular Biology and Microbiology from Mangalayatan University, Aligarh, Uttar Pradesh, India. He has worked prominently in the fields of plant biotechnology, molecular biology, immuno-informatics and microbiology. Surendra Prakash Gupta (PhD) is an Associate Professor in the Department of Biotechnology at Shri Vaishnav Institute of Sciences. He received a BSc Hons in Chemistry (2002) from Dayalbagh Educational Institute, Agra, India; an MSc (2006) and MTech (2008) in Biotechnology from C.C.S University, Meerut, India and Uttarakhand Technical University, India, respectively; and a PhD (2015) in Biological Science from Dayalbagh Educational Institute. He has 16 years of academic experience under various university roles. His research areas include structural, molecular, and computational biology and the development of nano-composite materials. Dr Gupta qualified for GATE in 2006 in Life Science. He has published five research papers in international peer-reviewed journals, 14 book chapters, five conference papers and more than 30 conference abstracts. Recently, he has been recognized as an NPTEL (National Programme on Technology Enhanced Learning) motivated learner and discipline star in the domain of Biosciences at the Indian Institute of Technology Kanpur. He has supervised BTech and MSc Biotechnology students successfully and currently supervises two PhD candidates. He is also a member of several reputed professional bodies such as the International Association of Engineers, Education and Development Research, and a Lifetime Member of the Indian Academicians and Researchers Association and the Indian Society for Training and Development, Indore.