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CRISPR-Cas Methods

M. Tofazzal Islam
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      This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
      Format: CONTRIBUTORS: M. Tofazzal Islam EAN: 9781071606186 COUNTRY: United States PAGES: WEIGHT: 566 g HEIGHT: 254 cm
      PUBLISHED BY: Springer-Verlag New York Inc. DATE PUBLISHED: 2020-07-05 CITY: GENRE: SCIENCE / Life Sciences / Genetics & Genomics WIDTH: 178 cm SPINE:

      Book Themes:

      Genetics (non-medical)

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      This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
      Format: CONTRIBUTORS: M. Tofazzal Islam EAN: 9781071606186 COUNTRY: United States PAGES: WEIGHT: 566 g HEIGHT: 254 cm
      PUBLISHED BY: Springer-Verlag New York Inc. DATE PUBLISHED: 2020-07-05 CITY: GENRE: SCIENCE / Life Sciences / Genetics & Genomics WIDTH: 178 cm SPINE:

      Book Themes:

      Genetics (non-medical)

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